We will examine infection with a second generation, barcoded ZIKV strain, based on PRVABC59. Four rhesus macaques (2 males, 2 females) will be infected with a second generation barcoded ZIKV clone, generated by Greg Ebel’s group at Colorado State University (CSU). This experiment will help characterize the in vivo growth characteristics and pathology of barcoded ZIKV clones in an immune competent NHP host.
Animals in this study will be subjected to mosquito feeding by Aedes aegypti (Black-eyed Liverpool strain) at 4 dpi to test transmissibility of barcoded clone version 2.0 from Indian rhesus macaques to mosquitoes.
Four Indian-origin rhesus macaques, 2 males and 2 females, will be infected subcutaneously with 10E4 PFU ZIKV Puerto Rico (PRVABC59) barcoded clone version 2.0. Blood will be collected from each animal at each time point (0-14, 21, 28 dpi) and samples will be sequenced to examine changes in the barcoded virus population over time. Viral RNA will be measured using qRT-PCR for all samples. The viral load trajectories and viremias of these animals will be compared with previous WT PRVABC59 infections and barcode version 1.0 (ZIKV-022).
This study is scheduled to begin in August 2017.
- Animals were challenged on August 7, 2017 with 10E4 PFU ZIKV Puerto Rico (PRVABC59) barcoded clone version 2.0.