To examine whether immunity elicited by previous Zika virus infection impacts subsequent infection with Dengue virus, we will infect three rhesus macaques that were previously infected with and re-challenged with French Polynesian Zika virus (ZIKV-FP) in our first Zika virus infections (ZIKV-001), with Dengue virus serotype 2 (DENV-2) strain New Guinea C.
Three Indian-origin rhesus macaques (ZIKV-001), previously infected with ZIKV-FP, will be infected subcutaneously with 105 PFU DENV-2 (New Guinea C strain). Blood plasma, urine, and saliva will be collected at multiple times during infection. Viral RNA will be measured using qRT-PCR for all sample types. Complete blood counts, chemistry panels, and immunophenotyping will be performed for all animals.
- February 6, 2017: challenge with DENV-2 was successful.
Clinical and Assay Data
Viral RNA quantification
- Neutralizing Antibody Titers
- PRNT90 ZIKA | DENV
- PRNT ZIKA | DENV
- DENV and ZIKV PRNT post ZIKV infection and pre DENV infection Graph | Data