Study Overview 
Investigator: O'Connor and Osorio Grant: NIH P51 to WNPRC and NIH R01 supplement 3R01AI116382-01A1S1 to O'Connor


ZIKV-024 tracks data in 37 datasets over 703 time points. Data is present for 3 Participants.

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ZIKV-024 

Primary objective:

To examine whether immunity elicited by previous Zika virus infection impacts subsequent infection with Dengue virus, we will infect three rhesus macaques that were previously infected with and re-challenged with French Polynesian Zika virus (ZIKV-FP) in our first Zika virus infections (ZIKV-001), with Dengue virus serotype 2 (DENV-2) strain New Guinea C.

Study design:

Three Indian-origin rhesus macaques (ZIKV-001), previously infected with ZIKV-FP, will be infected subcutaneously with 105 PFU DENV-2 (New Guinea C strain). Blood plasma, urine, and saliva will be collected at multiple times during infection. Viral RNA will be measured using qRT-PCR for all sample types. Complete blood counts, chemistry panels, and immunophenotyping will be performed for all animals.

Participants:

Updates:

  • February 6, 2017: challenge with DENV-2 was successful.

Clinical and Assay Data

Viral RNA quantification

Clinical

Immunology

  • Neutralizing Antibody Titers
    • PRNT90 ZIKA | DENV
    • PRNT ZIKA | DENV
    • DENV and ZIKV PRNT post ZIKV infection and pre DENV infection Graph | Data


Plasma DENV viral loads 

Pan urine DENV viral loads 

DENV and ZIKV neutralizing antibodies prior to DENV infection 

 

 

Neutralizing antibody data looking at the cross-reactivity of ZIKV and DENV prior to DENV-2 infection. DENV Data | ZIKV Data

  Attached Files  
   
 ZIKV-24_ZIKV_DENV2_d0_labkeyids.jpg


DENV neutralizing antibodies pre and post DENV-2 infection 

DENV neutralizing antibodies pre and post DENV-2 infection Data

  Attached Files  
   
 ZIKV024_DENVPRNT.jpg


ZIKV neutralizing antibodies prior and post DENV infection 

Neutralizing antibody data looking at the cross-reactivity of ZIKV and DENV. Animals were previous exposed to ZIKV-FP approximately one year prior to DENV-2 challenge.  Days post DENV-2 infection (ZIKV-024) indicated by "d". Data

  Attached Files  
   
 ZIKV-24_ZIKVPRNT.jpg


DENV Neutralizing Antibody Titers 

ZIKV Neutralizing Antibody Titers 

Dengue virus sequence 

We deep sequenced the dengue virus stock using the unbiased approaches.  This method converts the vRNA into double stranded cDNA.  The cDNA is fragmented with the Nextera XT kit and then tagged for sequencing on the Illumina MiSeq.  Data was analyzed using the Zequencer tool, but mapping to the dengue reference FJ39039.gbk.


The vcf file and an excel annotation file are attached.  

  Attached Files  
   
 Denv_20170125-R1-31163.mapping.annotated.vcf
 Denv_20170125_MA_AnnotationTable.csv