Study Overview 
Investigator: O'Connor and Osorio Grant: NIH P51 to WNPRC and NIH R01 supplement 3R01AI116382-01A1S1 to O'Connor


ZIKV-020 tracks data in 32 datasets over 610 time points. Data is present for 8 Participants.

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ZIKV-020 

Primary objectives

We will examine ZIKV tissue tropism and the duration of ZIKV RNA detection in maternal tissues at three time points following first trimester ZIKV infection: 30, 65 and 105 days post-infection. This specific study, ZIKV-020, will examine ZIKV tissue tropism and the duration of ZIKV RNA detection at 105 days post-infection. We will also examine ZIKV tissue tropism in fetal tissues at the same time points.

Study design

Pregnant Indian-origin rhesus macaques will be challenged subcutaneously at approximately day 45 of gestation with 10E4 PFU ZIKV Puerto Rico (PRVABC59). Plasma, urine and saliva viral loads will be measured frequently immediately following infection, then weekly until pregnancy termination. CSF viral loads will be measured twice during pregnancy. Amniotic fluid viral loads will be measured once. The dam will undergo necropsy at 105 days post-ZIKV infection and multiple tissues will be evaluated for ZIKV RNA and infectious virus. Fetal tissues will also be evaluated for ZIKV RNA and infectious virus.

Participants

  • 664184 was challenged with 10E4 PFU ZIKV-Puerto Rico strain subcutaneously on 8.18.2017, at 46 days of gestation
  • 416597 fetus of 664184
  • 484880 was infected with 10E4 PFU ZIKV-Puerto Rico strain subcutaneously on 5.30.17, at 43 days of gestation
  • 424847 fetus of 484880
  • 795784 was infected with 10E4 PFU ZIKV-Puerto Rico strain subcutaneously on 7.11.17, at 41 days of gestation
  • 499874 fetus of 795784
  • 918724 was infected with 10E4 PFU ZIKV-Puerto Rico strain subcutaneously on 7.28.17, at 50 days of gestation
  • 527421 fetus of 918724

Clinical and Assay Data

Viral RNA quantification

  • Plasma viral loads Chart
  • Pan urine viral loads Chart
  • CSF viral loads Chart

Clinical

Immunology

  • Neutralizing Antibody Titers
    • PRNT90 Titers Chart | /Data
    • PRNT Graph | Data

Ultrasound measurements

Updates

  • December 15, 2016: One macaque is in breeding currently.
  • 484880 is scheduled to be challenged on May 30, 2017 with 10E4 PFU ZIKV Puerto Rico (PRVABC59) via subcutaneous injection.
  • May 30, 2017: 484880 was challenged with 10e4 PFU ZIKV PR at 43 days of gestation.
  • June 2, 2017: The challenge was successful, 484880 has a positive plasma viral load at 1 DPI, quantification coming soon.
  • 484880 reached peak plasma viremia at 4 days post infection with a plasma viral load of 1,430,000.0
  • June 30, 2017: 795784, a pregnant dam, was added to the study and is scheduled to be challenged with 10E4 PFU ZIKV Puerto Rico on July 11, 2017 at 41 days of gestation.
  • July 12, 2017: 795784 had a positive plasma viral load at 1 DPI, quantification coming soon.
  • July 28, 2017: 918724 was challenged with 10e4 PFU ZIKV PR at 50 days of gestation.
  • August 15, 2017: The fetus of animal 918724 developed hydrops as demonstrated by subcutaneous edema and pericardial effusion on ultrasound a week ago, so a parvovirus PCR was tested on maternal blood and it was negative. On August 22, the hydrops looked as if it had resolved on ultrasound.
  • August 18, 2017: 664184 was challenged with 10e4 PFU ZIKV PR at 46 days of gestation.
  • September 19, 2017: 484880 underwent a C-section and an infant male macaque was born. No apparent birth defects were noted on physical exam. This infant will undergo neurobehavioral, hearing, vision and brain imaging assessments during its first week of life.


Visual Study Design 

  Attached Files  
   
 ZIKV-020_visual.jpeg


Plasma viral load 

Pan urine viral load 

CSF viral load