- Determine whether saliva transmission of Zika virus is possible via several mucosal routes.
One animal was challenged with a high dose (10e6 PFU) of the Asian lineage virus via tonsillar challenge to determine if mucosal infection is possible. Animal will be challenged up to 5 times (every 3 days) if not infected after the first challenge. We are sampling daily for up to 18dpi, then weekly through 28dpi. Viral loads will be performed on plasma, saliva oral swabs, urine, and whole blood. Serum will be collected for plaque assays/TCID50 once plasma viremia is detected.
One male rhesus macaque was challenged on August 15, 2016 with 10e6 PFU of the Asian lineage virus via tonsillar challenge. Similar to ZIKV-007
, this animal had a slight delay in peak viremia following challenge. After peak, viremia resolved to below the limit of detection by day 10. As in ZIKV-007
, this suggests that Zika infection is theoretically possible to transmit via a mucosal route.
- 511690 challenged with 10E6 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
Clinical and Assay Data
Viral RNA quantification
- Plasma viral loads Chart
- Urine viral loads Chart
- Oral swab viral loads Chart
- Neutralizing Antibody Titers