- Determine whether saliva transmission of Zika virus is possible via several mucosal routes.
Three recipient animals were challenged with donor saliva from ZIKV-008
. Saliva was applied to either the tonsils, conjunctiva, or intranasal mucosa. Recipient animals were challenged with donor saliva for 8 consecutive days starting on day 3 post-infection of the donor animal. We are sampling daily for up to 18dpi, then weekly through 28dpi. Viral loads will be performed on plasma, saliva oral swabs, urine, and whole blood. Serum will be collected for plaque assays/TCID50 once plasma viremia is detected.
Three animals were challenged on July 23, 2016 with donor saliva from oral swabs eluted in viral transport medium (ZIKV-011) applied to the tonsils, conjunctiva, or intranasal mucosa. Animal 678776 was challenged via tonsils, and a very low level blip was detected on day 2. A retest of this timepoint was negative for viral RNA. Additionally, the animal did not seroconvert, so it is unlikely that the animal was ever infected. No viremia was detected in the other recipient animals.
Clinical and Assay Data
Viral RNA quantification
- Plasma viral loads Chart
- Pan urine viral loads Chart
- Oral swab virus load Chart
- Neutralizing Antibody Titers