Study Overview 
Investigator: O'Connor and Osorio Grant: NIH P51 to WNPRC and NIH R01 supplement 3R01AI116382-01A1S1 to O'Connor


ZIKV-008 tracks data in 34 datasets over 459 time points. Data is present for 1 Participant.

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Study Navigator

ZIKV-008 

Primary objectives

  • Determine whether saliva transmission of Zika virus is possible via several mucosal routes.

Study design

One animal was challenged with 10e4 PFU of the Asian lineage virus subcutaneously, as in our previous studies. Oral swabs from this donor animal were eluted in saline to use for saliva challenges in three additional animals (ZIKV-009), with saliva applied to either the tonsils, conjunctiva, or intranasal mucosa. We are sampling daily for up to 18dpi, then weekly through 28dpi. Viral loads will be performed on plasma, saliva oral swabs, urine, and whole blood. Serum will be collected for plaque assays/TCID50 once plasma viremia is detected.

Results summary

Animal was successfully infected as in our previous studies after subcutaneous challenge with 10e4 PFU of the Asian lineage virus. Highest titer of ZIKV RNA isolated from oral swabs was just over 2,000 copies/ml. While infection was shown to be possible in ZIKV-007, it is likely that the saliva viral load in this donor was not nearly sufficient to infect the recipients in ZIKV-009.

Participants

  • 448436 challenged subcutaneously with 10E4 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1

Clinical and Assay Data

Viral RNA quantification

  • Plasma viral loads Chart
  • Pan urine viral loads Chart
  • Oral swab virus load Chart

Clinical

Immunology

  • Neutralizing Antibody Titers


Plasma Viral Load 

Oral swab viral load 

Pan urine viral load