- Determine whether saliva transmission of Zika virus is possible via several mucosal routes.
One animal was challenged with 10e4 PFU of the Asian lineage virus subcutaneously, as in our previous studies. Oral swabs from this donor animal were eluted in saline to use for saliva challenges in three additional animals (ZIKV-009
), with saliva applied to either the tonsils, conjunctiva, or intranasal mucosa. We are sampling daily for up to 18dpi, then weekly through 28dpi. Viral loads will be performed on plasma, saliva oral swabs, urine, and whole blood. Serum will be collected for plaque assays/TCID50 once plasma viremia is detected.
Animal was successfully infected as in our previous studies after subcutaneous challenge with 10e4 PFU of the Asian lineage virus. Highest titer of ZIKV RNA isolated from oral swabs was just over 2,000 copies/ml. While infection was shown to be possible in ZIKV-007
, it is likely that the saliva viral load in this donor was not nearly sufficient to infect the recipients in ZIKV-009
- 448436 challenged subcutaneously with 10E4 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
Clinical and Assay Data
Viral RNA quantification
- Plasma viral loads Chart
- Pan urine viral loads Chart
- Oral swab virus load Chart
- Neutralizing Antibody Titers