Study Overview 
Investigator: O'Connor and Osorio Grant: NIH P51 to WNPRC and NIH R01 supplement 3R01AI116382-01A1S1 to O'Connor


ZIKV-007 tracks data in 34 datasets over 460 time points. Data is present for 1 Participant.

Study Navigator
Study Navigator

ZIKV-007 

Primary objectives

  • Determine whether saliva transmission of Zika virus is possible via several mucosal routes.

Study design

One animal was challenged with a high dose (10e6 PFU) of the Asian lineage virus via tonsillar challenge to determine if mucosal infection is possible. We are sampling daily for up to 18dpi, then weekly through 28dpi. Viral loads will be performed on plasma, saliva oral swabs, urine, and whole blood. Serum will be collected for plaque assays/TCID50 once plasma viremia is detected.

Result summary

One male rhesus macaque was challenged on July 20, 2016 with 10e6 PFU of the Asian lineage virus via tonsillar challenge. This animal showed low levels of viremia on days 1 and 2 post-infection, which increased to over 1 million copies/ml of plasma by day 5. After peak, viremia resolved to below the limit of detection by day 14 similar to what we have seen in previous Zika challenges. This suggests that Zika infection is theoretically possible to transmit via a mucosal route.

Participants

  • 664817 challenged via tonsils with 10E6 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1

Clinical and Assay Data

Viral RNA quantification

  • Plasma viral loads Chart
  • Pan urine viral loads Chart
  • Oral Swab viral loads Chart

Clinical

Immunology

  • Neutralizing Antibody Titers


Viral Loads - Challenge Reservoir 

Pan urine viral load