- Determine whether saliva transmission of Zika virus is possible via several mucosal routes.
One animal was challenged with a high dose (10e6 PFU) of the Asian lineage virus via tonsillar challenge to determine if mucosal infection is possible. We are sampling daily for up to 18dpi, then weekly through 28dpi. Viral loads will be performed on plasma, saliva oral swabs, urine, and whole blood. Serum will be collected for plaque assays/TCID50 once plasma viremia is detected.
One male rhesus macaque was challenged on July 20, 2016 with 10e6 PFU of the Asian lineage virus via tonsillar challenge. This animal showed low levels of viremia on days 1 and 2 post-infection, which increased to over 1 million copies/ml of plasma by day 5. After peak, viremia resolved to below the limit of detection by day 14 similar to what we have seen in previous Zika challenges. This suggests that Zika infection is theoretically possible to transmit via a mucosal route.
- 664817 challenged via tonsils with 10E6 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
Clinical and Assay Data
Viral RNA quantification
- Plasma viral loads Chart
- Pan urine viral loads Chart
- Oral Swab viral loads Chart
- Neutralizing Antibody Titers