Study Overview 
Investigator: O'Connor and Osorio Grant: NIH P51 to WNPRC and NIH R01 supplement 3R01AI116382-01A1S1 to O'Connor


ZIKV-004 tracks data in 34 datasets over 541 time points. Data is present for 3 Participants.

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ZIKV-004: Infection of three rhesus macaques 

Primary objectives

  • Assess the infectivity of a Zika virus isolate from Asia at different doses in rhesus macaques
  • Measure concentration of Zika virus RNA in plasma, urine, CSF, saliva, and feces

Study design

Result summary

This study started Monday, March 28, 2016

Participants

  • 411359 challenged with 10E4 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
  • 181856 challenged with 10E5 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
  • 610107 challenged with 10E6 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
Real-time editorial study commentary

Clinical and Assay Data

Viral RNA quantification

  • Plasma viral loads Chart
  • Pan urine viral loads Chart
  • Cystocentisis viral loads Chart
  • CSF viral loads Chart
  • Oral swab viral loads Chart
  • Vaginal swab viral loads Chart
  • Serum viral loads Chart

Clinical

Immunology


Key data: plasma viral loads 

Neutralizing Antibody Titers 

ELISPOT Results 

We performed IFNg-ELISPOT at 4dpi, 10dpi, and 14dpi using peptide pools spanning the NS5 peptide for the Asian lineage zika virus. Each pool was comprised of 10 overlapping 15mer peptides. Each peptide pool was run in duplicate, as well as a Concanavalin A (ConA) positive control and a non-stimulated negative control. Data was baseline corrected by subtracting the average negative control values from each response. A threshold of 10.0 SFC/100,000 cells was set as the minimum value to be considered a positive T cell response.

411359     181856     610107

Shared responses were observed in animals with a shared MHC haplotype, which suggests that these peptide responses may be restricted the shared Mamu-A*004, Mamu-A*023, or Mamu-B*012b allele.

ZIKV_004_SUMMARY

  Attached Files  
   
 181856_ELISPOT.png
 610107_ELISPOT.png
 ZIKV_004_ELISPOT_SUMMARY.png
 411359_ELISPOT.png
 ZIKV_004_ELISPOT_DATA.xlsx


ZIKV neutralizing antibodies 

Neutralizing antibody data 28 DPI. Data

  Attached Files  
   
 ZIKV-004_nAb_graph_labkeyids.jpg