Investigator: O'Connor and Osorio Grant: NIH P51 to WNPRC and NIH R01 supplement 3R01AI116382-01A1S1 to O'Connor

ZIKV-004 tracks data in 34 datasets over 548 time points. Data is present for 3 Participants.

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Primary objectives

  • Assess the infectivity of a Zika virus isolate from Asia at different doses in rhesus macaques
  • Measure concentration of Zika virus RNA in plasma, urine, CSF, saliva, and feces

Study design

Result summary

This study started Monday, March 28, 2016


  • 411359 challenged with 10E4 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
  • 181856 challenged with 10E5 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
  • 610107 challenged with 10E6 PFU Zika virus/H.sapiens-tc/FRA/2013/FrenchPolynesia-01_v1c1
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Clinical and Assay Data

Viral RNA quantification

  • Plasma viral loads Chart
  • Pan urine viral loads Chart
  • Cystocentisis viral loads Chart
  • CSF viral loads Chart
  • Oral swab viral loads Chart
  • Vaginal swab viral loads Chart
  • Serum viral loads Chart



We performed IFNg-ELISPOT at 4dpi, 10dpi, and 14dpi using peptide pools spanning the NS5 peptide for the Asian lineage zika virus. Each pool was comprised of 10 overlapping 15mer peptides. Each peptide pool was run in duplicate, as well as a Concanavalin A (ConA) positive control and a non-stimulated negative control. Data was baseline corrected by subtracting the average negative control values from each response. A threshold of 10.0 SFC/100,000 cells was set as the minimum value to be considered a positive T cell response.

411359     181856     610107

Shared responses were observed in animals with a shared MHC haplotype, which suggests that these peptide responses may be restricted the shared Mamu-A*004, Mamu-A*023, or Mamu-B*012b allele.


  Attached Files  

Neutralizing antibody data 28 DPI. Data

  Attached Files